mouse stem cell factor (R&D Systems)
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mouse stem cell factor
Mouse Stem Cell Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+stem+cell+factor/Recombinant+Mouse+SCF+Protein/pmc12999497-385-33-38
Average 95 stars, based on 145 article reviews
Mouse Stem Cell Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+stem+cell+factor/Recombinant+Mouse+SCF+Protein/pmc12999497-385-33-38
Average 95 stars, based on 145 article reviews
mouse stem cell factor - by Bioz Stars,
2026-09
95/100 stars
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Recombinant:Article Title: Granulopoietic Dysregulation in a Patient-Tailored Mouse Model of Barth Syndrome Article Snippet: .. Hematopoietic clonogenic progenitors from 4-week-old wt♂ and Taz D75H ♂ bone marrow cells flushed from femurs were grown in 1% methylcellulose culture medium with 0.1 mM hemin (Sigma-Aldrich), 30% FBS, 1 U/mL recombinant human erythropoietin (rhEPO; Amgen), 50 ng/mL Article Title: Hematopoietic stem cell gene therapy ameliorates CNS involvement in murine model of GM1-gangliosidosis Article Snippet: Whole bone marrow cells were harvested from the tibias and femurs of donor βgal −/− mice (8–12 weeks old) and concentrated HSCs using a lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). .. Lineage-negative cells were transduced at a MOI of 50, with LV carrying cGLB1 (LV-GLB1) or EGFP (LV-GFP) genes for 24 h on a RetroNectin-coated 6-well plate (Takara Bio, Shiga, Japan) at a density of 2 × 10 6 cells/mL in Dulbecco’s modified Eagle’s medium (DMEM; Fujifilm, Wako Pure Chemical, Tokyo, Japan) with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA), recombinant mouse Flt-3 ligand (rnFlt3, 100 ng/mL; R&D Systems, Minneapolis, MN), and Article Title: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells. Article Snippet: Cell concentration was determined with a Cellometer (Nexcelom Biosciences) and 200,000 cells were plated in in low-adhesion 24-well plates (Costar) in 400 μL sphere media. .. Sphere media consisted of DMEM-F12/1% penicillin-streptomycin, 10 μg/mL ITS, 1% N2, (Gibco); 1 μM dexamethasone, 20 ng/mL FGF2, 20 ng/mL EGF, 100 ng/mL FGF10, 100 ng/mL Article Title: In vitro production of red blood cells with sortaggable proteins Article Snippet: .. Immediately following spin-infection, the virus-containing supernatant was aspirated and replaced with erythroid maintenance medium (StemSpan-SFEM (StemCell Technologies) supplemented with Article Title: Globin vector regulatory elements are active in early hematopoietic progenitor cells Article Snippet: Donor bone marrow was flushed from the femurs of 8- to 16-week-old C57BL/6 (CD45.1, Jax) or Hbbth3/+ male mice that had been injected intraperitoneally (i.p.) 6 days earlier with 5-fluorouracil (5-FU, Pharmacia; 150 mg/kg body weight). .. Bone marrow cells were resuspended in serum-free X-VIVO10 medium (Lonza, Basel, Switzerland), and supplemented with Article Title: Method of preparing hematopoietic stem and progenitor cells for transplantation Article Snippet: Bone marrow cells were harvested from two tibias by flushing with DPBS (HYCLONE™) Splenocytes were isolated from spleens by homogenizing individual spleens through a 70-pM mesh (Fisher) using DPBS. .. Colony forming units (CFU)-granulocyte macrophage, burst forming units (BFU)-erythroid (E), granulocyte, erythrocyte, macrophage, and megakaryocyte (GEMM) colonies were scored after 7 days in culture by plating whole bone marrow at 5×104 cells/mL and splenocytes at 2×105 cells/mL in 1% methylcellulose/Iscoves Modified Dulbeccos Medium (IMDM) with 30% FBS (Corning), 1 U/mL erythropoietin (Amgen), 5% (v/v) pokeweed mitogen spleen cell conditioned medium (PWMSCM), 50 ng/mL Article Title: DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Gαi signaling Article Snippet: .. BM Lin- cells were cultured in RPMI1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/mL Article Title: PD-L1-Binding Antigen Presenters: Redirecting Vaccine-Induced Antibodies for Cancer Immunotherapy. Article Snippet: 4T1 cells were cultured in RPMI-1640 medium with 10% FBS, 2 mM l-glutamine (Invitrogen) and 1% penicillin-streptomycin (Invitrogen). .. KIL C.2 cells were cultured in PriGrow V medium (Applied Biological Materials) supplemented with 30% fetal bovine serum (Sigma Aldrich), 2 mM L-glutamine, 50 ng/mL Modification:Article Title: Hematopoietic stem cell gene therapy ameliorates CNS involvement in murine model of GM1-gangliosidosis Article Snippet: Whole bone marrow cells were harvested from the tibias and femurs of donor βgal −/− mice (8–12 weeks old) and concentrated HSCs using a lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). .. Lineage-negative cells were transduced at a MOI of 50, with LV carrying cGLB1 (LV-GLB1) or EGFP (LV-GFP) genes for 24 h on a RetroNectin-coated 6-well plate (Takara Bio, Shiga, Japan) at a density of 2 × 10 6 cells/mL in Dulbecco’s modified Eagle’s medium (DMEM; Fujifilm, Wako Pure Chemical, Tokyo, Japan) with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA), recombinant mouse Flt-3 ligand (rnFlt3, 100 ng/mL; R&D Systems, Minneapolis, MN), and Article Title: Method of preparing hematopoietic stem and progenitor cells for transplantation Article Snippet: Bone marrow cells were harvested from two tibias by flushing with DPBS (HYCLONE™) Splenocytes were isolated from spleens by homogenizing individual spleens through a 70-pM mesh (Fisher) using DPBS. .. Colony forming units (CFU)-granulocyte macrophage, burst forming units (BFU)-erythroid (E), granulocyte, erythrocyte, macrophage, and megakaryocyte (GEMM) colonies were scored after 7 days in culture by plating whole bone marrow at 5×104 cells/mL and splenocytes at 2×105 cells/mL in 1% methylcellulose/Iscoves Modified Dulbeccos Medium (IMDM) with 30% FBS (Corning), 1 U/mL erythropoietin (Amgen), 5% (v/v) pokeweed mitogen spleen cell conditioned medium (PWMSCM), 50 ng/mL Virus:Article Title: In vitro production of red blood cells with sortaggable proteins Article Snippet: .. Immediately following spin-infection, the virus-containing supernatant was aspirated and replaced with erythroid maintenance medium (StemSpan-SFEM (StemCell Technologies) supplemented with Cell Culture:Article Title: Globin vector regulatory elements are active in early hematopoietic progenitor cells Article Snippet: Donor bone marrow was flushed from the femurs of 8- to 16-week-old C57BL/6 (CD45.1, Jax) or Hbbth3/+ male mice that had been injected intraperitoneally (i.p.) 6 days earlier with 5-fluorouracil (5-FU, Pharmacia; 150 mg/kg body weight). .. Bone marrow cells were resuspended in serum-free X-VIVO10 medium (Lonza, Basel, Switzerland), and supplemented with Article Title: DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Gαi signaling Article Snippet: .. BM Lin- cells were cultured in RPMI1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/mL Article Title: PD-L1-Binding Antigen Presenters: Redirecting Vaccine-Induced Antibodies for Cancer Immunotherapy. Article Snippet: 4T1 cells were cultured in RPMI-1640 medium with 10% FBS, 2 mM l-glutamine (Invitrogen) and 1% penicillin-streptomycin (Invitrogen). .. KIL C.2 cells were cultured in PriGrow V medium (Applied Biological Materials) supplemented with 30% fetal bovine serum (Sigma Aldrich), 2 mM L-glutamine, 50 ng/mL Chemotaxis Assay:Article Title: DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Gαi signaling Article Snippet: .. BM Lin- cells were cultured in RPMI1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/mL |